Coding
Part:BBa_K5228000:Design
Designed by: Narjis Alhusseini Group: iGEM24_UBC-Vancouver (2024-09-01)
Thermostable Terminal Deoxynucleotidyl Transferase (ThTdT)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 275
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 275
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 275
Illegal BglII site found at 164
Illegal BglII site found at 431
Illegal BglII site found at 799 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 275
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 275
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
TBD
Source
Derived from literature which generated a thermostable TdT mutant