Coding

Part:BBa_K5228000:Design

Designed by: Narjis Alhusseini   Group: iGEM24_UBC-Vancouver   (2024-09-01)


Thermostable Terminal Deoxynucleotidyl Transferase (ThTdT)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 275
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 275
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 275
    Illegal BglII site found at 164
    Illegal BglII site found at 431
    Illegal BglII site found at 799
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 275
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 275
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TBD


Source

Derived from literature which generated a thermostable TdT mutant

References